Illumina NGS Sample Prep Automation from Hamilton0 pages

Illumina NGS Sample Prep
Automation from Hamilton
®

Hamilton and Illumina are working together to qualify the TruSeq® and Nextera® series of sample preparation kits for use on the
Microlab® STAR™. The methods include the following features:
XX automation, with on-deck incubations and plate shaking, plate
Full
stacking, and large tip inventory for maximum walk-away time
XX to 96 samples processed in a single run
1
XX
Easy-to-use graphical interface
XX
Barcode verification for proper placement of reagents, plates, and tips
XX
Fully automated magnetic-bead based cleanup and size selection steps
XX
Offline PCR thermal cycling, to minimize system cost
XX
Automated error handling
XX
Existing methods can be customized to meet each laboratory’s needs
and budget

The methods currently available for Illumina sample preparation are:
XX
TruSeq Stranded mRNA*
XX
TruSeq Stranded Total RNA

Sample Prep Automation
Available from Hamilton:

Distinct Processes

XX
Magnetic bead or vacuum based cleanup

Choose one or more of these 4 processes, to suit your workflow

XX
Accurate small-volume pipetting
XX
On-deck incubations
XX
On-deck orbital plate shaking

TruSeq Stranded mRNA

TruSeq Stranded Total RNA

1 - Purify, Fragment, and Synthesize 1 - Deplete, Fragment, and Synthesize
2 - Adenylate and Ligate

2 - Adenylate and Ligate

3 - PCR Amplification

3 - PCR Amplification

4 - Normalize and Pool

4 - Normalize and Pool

XX
Optional integration with third-party
sealers and thermal cyclers
XX
Barcode reading for process security
and sample tracking
XX
Application support for method
customization