Corning® Gentest? Fresh Human Hepatocytes0 pages

Corning® Gentest™
Fresh Human Hepatocytes
Hepatocytes in plates, sandwich culture, and suspension

Corning offers a full portfolio of fresh hepatocytes in plates, sandwich
culture, and suspension to support drug metabolism and transport
studies. High-quality Corning Gentest fresh hepatocytes maintain
morphology and function for long-term assays, such as enzyme induction, toxicity, metabolism of stable compounds, and drug transporter
assays.
Corning Gentest hepatocytes cultured on Collagen I with a Corning
Matrigel® overlay in a sandwich culture form an in vivo-like hepatobiliary network that is critical for studying hepatic uptake and biliary
efflux transporter activity. Compared to Collagen I surfaces alone, the
Corning Matrigel overlay creates a more physiological-like environment, maintains hepatocyte-specific morphology, and improves basal
and induced CYP450 activities.

Features
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Supports ADME assays, such as metabolism, induction, transport,
and toxicity requiring cultured human plateable hepatocytes

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Supports short-term ADME assays in suspension, such as metabolic
stability and uptake transporter studies

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Saves researchers time with premade, ready-to-use hepatocyte
cultures on Collagen I-coated plates with, or without, Corning
Matrigel overlay

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Enables high throughput screening for discovery and development
of drug candidates

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Corning Collagen I substratum with Corning Matrigel overlay

t – Improves cell health with increased basal and induced CYP450

activity

Corning Matrigel Overlay Promotes a
More In Vivo-like Environment
The Corning Matrigel overlay improves basal activities
for CYP450 activities compared to the conventional
Collagen I coated surface.
Corning Matrigel overlay improves both basal and
induced CYP3A4 activity of human fresh hepatocytes
(Corning fresh hepatocyte lot 323 cultured on Corning
BioCoat Collagen I plates)

t – Supports bile canaliculi formation for efflux drug transport studies

t – Maintains typical hepatocyte morphology longer than a Collagen I

surface

Fresh hepatocytes can be cultured for more than one week, which
makes them ideal for studies requiring longer culture times, such as
induction, efflux transport, and toxicity assays.

Multiple Formats Provide Researchers with Flexibility
Corning’s portfolio of fresh hepatocytes includes flasks and 6, 12,
24, 48, and 96 well plates. These formats are available with Corning
BioCoat™ Collagen I as a substratum with, or without, Corning
Matrigel overlay.

CYP3A4 Basal

CYP3A4 Activity, pmol/min/mg

Fresh Hepatocytes Maintain Hepatic Morphology
and Function

CYP3A4 Activity in Corning Matrigel Overlay Sandwich Culture

CYP3A4 Induced

No Matrigel Overlay

With Matrigel Overlay