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Corning® Gentest? High Viability CryoHepatocyte Recovery Kit0 pages
Corning® Gentest™ High Viability
CryoHepatocyte Recovery Kit
The Corning Gentest high viability cryohepatocyte recovery kit
provides a high-viability, high-yield recovery medium optimized
for a single centrifugation step as well as an optimized plating
medium for high plating efficiency.
Features
w tImproves cryopreserved hepatocyte cell viability, yield, and
plating efficiency
w tSaves researchers time with an optimized single
centrifugation recovery step versus traditional two-step
processes
w tProvides recovery medium for two purifications; up to five
vials of cryopreserved hepatocytes can be processed per
purification
w tSupports human plateable cryopreserved hepatocyte recovery
and plating for induction assays or other assays requiring
plated cells such as metabolism, transporter, and toxicity
studies
Viability Improvement
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Tests on 25 lots of Corning Gentest inducible-qualified cryopreserved hepatocytes showed the Corning Gentest high viability
cryohepatocyte recovery kit achieved ≥ 80% post-thaw viability
for all lots. Recovery of viable cells also increased by an average
of 1.5 million cells/vial (24% increase) when compared to other
recovery and thawing medium.
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The non-cytotoxic nature of the recovery medium allows
researchers to use a one-step centrifugation step and move
directly to plating hepatocytes. The need for a second wash and
centrifugation step is eliminated.
Convenient format
The Corning Gentest high viability cryohepatocyte recovery kit
provides two tubes of post-thaw recovery medium and two
tubes of plating medium for high throughput screening or
pooled product applications.
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6
4
2
0
266
267
228
264
178
166
260
246
262
195
Avg.
Lot Number
Figure 1. Viability Improvement versus Conventional Recovery Medium.
Post-thaw viability increased 7% on average for a representative 10
Corning Gentest inducible cryopreserved hepatocyte lots when the
Corning Gentest high viability cryohepatocyte recovery medium was
compared to conventional recovery medium.
Recovery Improvement
Delta Recovery (M/vial)
Single recovery centrifugation step saves time
versus traditional two-step processes
Delta Viability (%)
Increases high post-thaw viability and yield
3.5
3.0
2.5
2.0
1.5
1.0
0.5
0.0
266
267
228
264
178
166
260
246
262
195
Avg.
Lot Number
Figure 2. Recovery Improvement versus Conventional Recovery Medium.
Post-thaw recovery increased 1.7 million cells/vial on average
(25% increase) for a representative 10 Corning Gentest inducible
cryopreserved hepatocyte lots when the Corning Gentest high viability
cryohepatocyte recovery medium was compared to conventional recovery medium.
