Corning® Gentest? Plateable Human CryoHepatocytes0 pages

Corning® Gentest™ Plateable Human
CryoHepatocytes

Corning Gentest plateable cryopreserved hepatocytes are prescreened, application-specific products designed to save researchers
time evaluating multiple lots. Each lot is characterized for transporter-mediated uptake and biliary efflux activity, high metabolic
activity for low clearance drugs, and enzyme induction. These characterizations provide researchers with the data necessary to select
lots which are well-suited to their research needs.

Features
w tSupports ADME assays such as drug metabolism, induction,

transporter, and toxicity studies
w tPromotes high viability, high recovery, and high plating efficiency

when using a combination of Corning Gentest plateable
hepatocytes and Corning Gentest high viability recovery and
plating medium
w tSupports long-term, multi-site studies with typical lots

>500 vials
w tTransporter-Qualified Hepatocytes:

t - emonstrate extensive bile canaliculi formation for assessing
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drug efflux transport when using a combination of Corning
Collagen I as the surface and a Corning Matrigel® overlay
t - upport expanding FDA-recommended studies for efflux (P-gp,
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BCRP) and uptake (OATP1B1, OATP1B3) transporters
w tMetabolism-Qualified Hepatocytes:

t - xtend time course of metabolic activity when using plateable
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human cryopreserved hepatocytes compared to cells in
suspension; ideal for evaluating the metabolic stability of slowly
metabolized test compounds
t - emonstrate high Midazolam clearance, consistently
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>0.2 mL/hr/million cells, and metabolite formation activity for
P450 and UGT enzymes
w tInducible-Qualified Hepatocytes:

t - atisfy FDA recommended fold induction for important P450s
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t - emonstrate physiologically relevant basal activity and fold
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induction when using a combination of Corning Gentest
hepatocytes, Corning Matrigel thin-coat surface, and Corning
Matrigel overlay compared to conventional Collagen I surfaces

Pre-qualification for plateable hepatocyte
applications helps researchers select hepatocytes
well-suited to their needs
Lots are qualified for transporter, metabolism, and induction
assays, and data is reported for relevant assays. This data provides researchers convenience and flexibility to select the best
lot(s) for their application(s). Note: Some lots may be suitable for
more than one assay type.

Characterization data provides insights into key
applications
High viability, high quality lots are characterized for their cell
morphology, bile canaliculi formation for studying hepatic
uptake and efflux transporters, metabolic activity for intrinsic
clearance measurement and metabolite formation, and drug
metabolizing enzyme induction. On average Corning Gentest
plateable cryopreserved hepatocyte lots demonstrate cell viabilities of ≥85%, with most lots having cell viabilities of ≥90%, and
confluency of ≥80%. High viability and superior morphological
attributes provide confidence that the cells have retained hepatic
functions.

Extensive supply of large lots provides consistent
data for multi-year studies and multiple sites
Corning provides researchers with an extensive selection of
plateable hepatocyte lots typically with >500 vials. These large
lots are ideal for long-term, multi-site projects, where reproducible data is desirable.