Corning® Supersomes? Human Carboxylesterase Portfolio0 pages

Corning® Supersomes™ Human
Carboxylesterase Portfolio
Human Recombinant Drug Metabolizing Enzymes

Human carboxylesterase 1 (hCES1) and carboxylesterase 2 (hCES2)
are members of the serine esterase superfamily, exhibiting broad
substrate specificity, and involved in xenobiotic and endobiotic
metabolism. Human CES1 and CES2 are primarily expressed in the
liver and small intestine. Human CES1 is expressed as two major
isoforms; hCES1b and hCES1c.
Corning Supersomes CES portfolio of human recombinant metabolizing enzymes match the published sequence from the U.S.
National Library of Medicine native DNA sequence and provide high
levels of enzymatic activity for efficient metabolite production.

Features
w tFacilitates efficient metabolite formation with long linear time

course; ideal for slow metabolizing drug compounds.
w tEnsures hydrolase activity of recombinant enzymes is functionally

similar to hCES1 and hCES2 expressed in human liver and small
intestine microsomes, respectively.
w tDelivers high catalytic activity, ideal for robust high-throughput

screening assays including drug-drug interaction studies.
w tSupports essential assays such as reaction phenotyping and

enzyme inhibition required for FDA new drug registration and
other pharmacokinetic studies appropriate for evaluating
pro-drugs and non-CYP pathways of elimination.
w tManufactured using baculovirus transfected insect cell expression

system.

High enzymatic activity
The Corning Supersomes product line is engineered to provide
efficient metabolite production with long linear metabolite formation with typical times of >30 minutes, making the products
highly suited to identifying metabolites for slowly metabolized
compounds.

Engineered for native activity
Corning’s CES1b, CES1c, and CES2 behave like native carboxylesterases expressed in human liver and intestine as demonstrated by measured hydrolase activity where the Vmax
and Km of recombinant hCES1b and hCES2 are similar to
those of human liver and small intestine microsomes (shown
in Figure 1).

Extensive portfolio of Phase 1
and Phase 2 recombinant enzymes
Corning Supersomes are recognized worldwide as the
“industry gold standard”, with the most extensive portfolio
of CYPs, UGTs, FMOs, MAOs, NATs, and now CESs.